Role of amaltas and dandasa in controlling biofilm formation of streptococcus mutans and pseudomonas

People usually give least importance to their oral hyiegene as a consequence they are suffering from so many disease due to oral biofilm that form over the teeth. Also when patient is going for dental procedures they were exposed to water from the dental lines of this unit. Pseudomonas aeroginosa were the main culprit present in these water line and form biofilm there as well as in host causing infection. Biofilm forming property confers virulence characteristics thus making such bacteria highly resistant to commonly used antibiotics. We analyzed the antibiofilm activity of natural occurring substances like dandasa [juglans regia] and amaltas [Cassia fistula]. Experimental study. All clinical isolates were identified at BUMDC and PNS microbiology lab [Karachi] by standard biochemical methods. This study was conducted between Nov 2012 to Feb 2013. 40 streptococcus mutan and 32 pseudomonas aerogenosa samples were taken from oral biofilm and contaminated denal lines water respectively and characterized using conventional biochemical, cultural and molecular methods. Biofilm forming activity of these isolates were checked. Then I mixed the dandasa and amaltas and observe the antibiofilm forming activity. Both dandasa and amaltas in a cone, of 12.5 mg/ml and 3.2 mg/ml respectively show good antibiofilm forming activity against pseudomonas aerogenosa and in a cone 0.8 mg/ml and 12.5 mg/ml respectively against streptococcus mutan. But combinations of dandasa with amaltas were found to be not more effective in inhibiting biofilm formation suggesting a indifferent activity with antiadhesive index of 1.0 and 0.75 against streptococcus mutans, and pseudomonas aerogenosa respectively. Streptococcus mutans in oral biofilm and pseudomonas in dental water lines exhibited biofilm formation which is the cause for antibiotic resistance and providing shelter to other organism. Amaltas and Dandasa provide a good antibiofilm activity individually

Antibiofilm forming activity of naturally occurring compound

It has been observed that people in general do not pay much attention to maintain proper oral hygiene as a result organisms which are a part of normal flora of oral cavity form oral biofilm over the teeth. When patient is catheterised mostly the catheter remain there for a long period especially in older immobilised very sick patients and over these catheter due to fecal contamination ecoli biofilm formed. Biofilm forming property confers virulence characteristics thus making such bacteria highly resistant to commonly used antibiotics. We analysed the antibiofilm activity of natural occurring substances like dandasa [cassia fistula] and green tea [Camellia sinensis]. Forty streptococcus mutans and 45 ecoli samples were taken from oral biofilm and contaminated urinary catheter respectively and characterised using conventional biochemical, cultural and molecular methods. The biofilm forming activity of these isolates using 96 well plates were checked. Then the dandasa and green tea with those biofilm formers were mixed and observed the antibiofilm forming activity. Both dandasa and green tea in a concentration of 6.2 mg/ml and 12.5 mg/ml respectively show good antibiofilm forming activity against streptococcus mutan and in a concentration of 12.5 mg/ml and 3.1 mg/ml respectively against E. coli. Combinations of dandasa with green tea were found to be not more effective in inhibiting biofilm formation suggesting in different activity with anti-adhesive index of 1.5 and 0.75 against Streptococcus mutans, and ecoli respectively. Streptococcus mutans in oral biofilm and ecoli in urinary catheter biofilm exhibited biofilm formation which is the cause for antibiotic resistance and providing shelter to other organism. Green tea and dandasa provide a good antibiofilm activity individually

Physical and microbiological analysis of water from different sources of Karachi

A number of diseases are caused by consuming water of poor quality. According to a community health study 30% of all reported cases of illnesses and 40% of deaths in Pakistan are due to waterborne disease. The purpose of the study was to i] to determine the physical quality and presence of total coliforms and Escherichia coli count and also ii] to compare the coliform and Excherichia coli bacteria in drinking water of different towns of Karachi with WHO's recommended values. Retrospective Study. This study was conducted in the Department of Biochemistry in collaboration with Department of Microbiology, Basic Medical Sciences Institute, Jinnah postgraduate medical Centre Karachi during the period of June 2007 to February 2008. Multiple water sample collected from 18 towns of Karachi by different sources like Piped water, hand pumps, Mineral bottles and Filter plants. The 250 milliliters sample for microbiological analysis was collected to perform all the required analyses and to provide for any quality control need, split samples or repeat examination. Statistical Package for social Science [SPSS version 11.0] used for data feeding and analysis. The results were given in the text as number and percentage for qualitative/ categorical variables like E. Coli and coliform and mean and standard deviation for quantitative variables like Physical parameters pH, Temp, DO, Conductivity, TDS]. The pH in tap water samples ranged between 6.8 to 7.6. Temperature recorded at spot ranged between 25- 32 [degree sign] c. The dissolved oxygen varied from 3.6 to 4.8 pm, its permissible limit is 4ppm. In 108 tap water samples MPN of coliform/100 ml water samples were < 10 in 21[19.4%] samples and > 10 in 87 [80.6%] samples. Recovery of E. coli as indicator organisms of fecal pollution in different water samples were found. During the drinking water quality assessment a wide range of pollutions were found and generated very useful baseline information on the current pollution status of supplied water to Karachi which indicates that Karachi is receiving highly polluted water